HDGF Antibody (5U7A7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15281
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5U7A7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HDGF (P51858). YQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNST
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HDGF Antibody (5U7A7)
Western Blot: HDGF Antibody (5U7A7) [NBP3-15281]
Western Blot: HDGF Antibody (5U7A7) [NBP3-15281] - Western blot analysis of extracts of various cell lines, using HDGF antibody (NBP3-15281) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunocytochemistry/ Immunofluorescence: HDGF Antibody (5U7A7) [NBP3-15281]
Immunocytochemistry/Immunofluorescence: HDGF Antibody (5U7A7) [NBP3-15281] - Immunofluorescence analysis of U2OS cells using HDGF Rabbit mAb (NBP3-15281) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunocytochemistry/ Immunofluorescence: HDGF Antibody (5U7A7) [NBP3-15281]
Immunocytochemistry/Immunofluorescence: HDGF Antibody (5U7A7) [NBP3-15281] - Immunofluorescence analysis of NIH/3T3 cells using HDGF Rabbit mAb (NBP3-15281) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for HDGF Antibody (5U7A7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HDGF
Long Name
Hepatoma-derived Growth Factor
Alternate Names
HuHGF
Gene Symbol
HDGF
Additional HDGF Products
Product Documents for HDGF Antibody (5U7A7)
Product Specific Notices for HDGF Antibody (5U7A7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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