HDGF Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71926
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 200 and 240 of human Hepatoma-Derived Growth Factor using the numbering given in entry NP_004485.1 (GeneID 3068).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HDGF Antibody
![Western Blot: HDGF Antibody [NBP1-71926]](https://resources.bio-techne.com/images/products/HDGF-Antibody-Western-Blot-NBP1-71926-img0001.jpg "Western Blot: HDGF Antibody [NBP1-71926]")
Western Blot: HDGF Antibody [NBP1-71926]
Western Blot: HDGF Antibody [NBP1-71926] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-HDGF antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HDGF was also immunoprecipitated by rabbit anti-HDGF antibodies NBP1-71924 and NBP1-71925, which recognize upstream epitopes.![Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926]](https://resources.bio-techne.com/images/products/HDGF-Antibody-Immunohistochemistry-Paraffin-NBP1-71926-img0007.jpg "Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926]")
Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926]
Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- HDGF used at a dilution of 1:1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).![Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926]](https://resources.bio-techne.com/images/products/HDGF-Antibody-Immunohistochemistry-Paraffin-NBP1-71926-img0006.jpg "Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926]")
Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926]
Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926] - Immunohistochemistry-Paraffin: HDGF Antibody [NBP1-71926] - FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-HDGF left image, middle image and right image used at a dilution of 1: 1,000 (1ug/ml) or 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).Applications for HDGF Antibody
Application
Recommended Usage
Immunohistochemistry
1:1000 - 1:5000
Immunohistochemistry-Paraffin
1:1000- 1:5000
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HDGF
Long Name
Hepatoma-derived Growth Factor
Alternate Names
HuHGF
Gene Symbol
HDGF
UniProt
Additional HDGF Products
Product Documents for HDGF Antibody
Product Specific Notices for HDGF Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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