HDJ2 Antibody (23C6)

Western Blot: HDJ2 Antibody (23C6) [NBP3-26616] -

Western Blot: HDJ2 Antibody (23C6) [NBP3-26616] - Positive Western Blot detected in: U251 whole cell lysate, Ntera-2 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate.
All lanes: HDJ2 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 45, 38 kDa
Observed band size: 45-55 kDa

Immunocytochemistry/Immunofluorescence: HDJ2 Antibody (23C6) [NBP3-26616] -

Immunocytochemistry/Immunofluorescence: HDJ2 Antibody (23C6) [NBP3-26616] - Staining of A549 cell with HDJ2 Antibody (23C6) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 550-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: HDJ2 Antibody (23C6) [NBP3-26616] -

Flow Cytometry: HDJ2 Antibody (23C6) [NBP3-26616] - Overlay Peak curve showing A549 cells stained with HDJ2 Antibody (23C6) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.