HELT Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90761
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for HELT Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SDKLKERKVSRLSPNGTCALVVEASDSPTRHLGGPMAGKCPHGTLSVEESRVRT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HELT Antibody
![Western Blot: HELT Antibody [NBP1-90761]](https://resources.bio-techne.com/images/products/HELT-Antibody-Western-Blot-NBP1-90761-img0005.jpg "Western Blot: HELT Antibody [NBP1-90761]")
Western Blot: HELT Antibody [NBP1-90761]
Western Blot: HELT Antibody [NBP1-90761] - Analysis in control (vector only transfected HEK293T lysate) and HELT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunohistochemistry: HELT Antibody [NBP1-90761]](https://resources.bio-techne.com/images/products/HELT-Antibody-Immunohistochemistry-NBP1-90761-img0003.jpg "Immunohistochemistry: HELT Antibody [NBP1-90761]")
Immunohistochemistry: HELT Antibody [NBP1-90761]
Immunohistochemistry: HELT Antibody [NBP1-90761] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.Applications for HELT Antibody
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HELT
Alternate Names
hairy and enhancer of split-related protein HELT, helt bHLH transcription factor, HES/HEY-like transcription factor, Hey-like transcription factor, Megane bHLH factor, Mgn
Gene Symbol
HELT
Additional HELT Products
Product Documents for HELT Antibody
Product Specific Notices for HELT Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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