Hemicentin 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30512

Novus Biologicals, part of Bio-Techne
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NBP2-30512
NBP2-30512-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Hemicentin 2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: FSTQPLLDLNHTLEWPLQGVPISLVINSTGLKAPGRLDSVELAQSSGKPLLTLPTKPLSNGSTHQLWGGPPFHTPKERFYLKVKGKDHE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Hemicentin 2 Antibody

Immunohistochemistry-Paraffin: Hemicentin 2 Antibody [NBP2-30512]

Immunohistochemistry-Paraffin: Hemicentin 2 Antibody [NBP2-30512]

Immunohistochemistry-Paraffin: Hemicentin 2 Antibody [NBP2-30512] - Staining in human small intestine and liver tissues using anti-HMCN2 antibody. Corresponding HMCN2 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Hemicentin 2 Antibody [NBP2-30512]

Immunocytochemistry/ Immunofluorescence: Hemicentin 2 Antibody [NBP2-30512]

Immunocytochemistry/Immunofluorescence: Hemicentin 2 Antibody [NBP2-30512] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining shown in green.
Immunohistochemistry-Paraffin: Hemicentin 2 Antibody [NBP2-30512]

Immunohistochemistry-Paraffin: Hemicentin 2 Antibody [NBP2-30512]

Immunohistochemistry-Paraffin: Hemicentin 2 Antibody [NBP2-30512] - Staining of human liver shows low expression as expected.
View 1 more images

Applications for Hemicentin 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hemicentin 2

The HMCN2 (Hemicentin 2) gene encodes a Hemicentin-2 protein that in isoform 1 is 5,065 amino acids long, 542 kDA, in isoform 2 is 4,198 amino acids long, 448 kDA, and in isoform 3 is 4,427 amino acids long at 474 kDA.

Alternate Names

hemicentin-2

Gene Symbol

HMCN2

UniProt

Q8NDA2 (Human)

Additional Hemicentin 2 Products

Product Documents for Hemicentin 2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Hemicentin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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