Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25499
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2457
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free
Immunogen
Synthetic peptide of human Hemoglobin epsilon.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free [NBP3-25499] -
Immunocytochemistry/Immunofluorescence: Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free [NBP3-25499] - Hemoglobin epsilon antibody [HL2457] detects Hemoglobin epsilon protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hemoglobin epsilon stained by Hemoglobin epsilon antibody [HL2457] (NBP3-25499) diluted at 1:500. Blue: Fluoroshield with DAPI .
Immunohistochemistry-Paraffin: Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free [NBP3-25499] -
Immunohistochemistry-Paraffin: Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free [NBP3-25499] - Hemoglobin epsilon antibody [HL2457] detects secreted Hemoglobin epsilon protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. Hemoglobin epsilon stained by Hemoglobin epsilon antibody [HL2457] (NBP3-25499) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free [NBP3-25499] -
Western Blot: Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free [NBP3-25499] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [HL2457] (NBP3-25499) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Applications for Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Hemoglobin epsilon
Alternate Names
epsilon globin, Epsilon-globin, HBEepsilon-globin, Hemoglobin epsilon chain, hemoglobin subunit epsilon, hemoglobin, epsilon 1
Gene Symbol
HBE1
Additional Hemoglobin epsilon Products
Product Documents for Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free
Product Specific Notices for Hemoglobin epsilon Antibody (HL2457) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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