Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25500
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2458
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free
Immunogen
Synthetic peptide of human Hemoglobin epsilon.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free
Western Blot: Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free [NBP3-25500] -
Western Blot: Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free [NBP3-25500] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [HL2458] (NBP3-25500) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Western Blot: Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free [NBP3-25500] -
Western Blot: Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free [NBP3-25500] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [HL2458] (NBP3-25500) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free [NBP3-25500] -
Immunohistochemistry-Paraffin: Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free [NBP3-25500] - Hemoglobin epsilon antibody [HL2458] detects secreted Hemoglobin epsilon protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. Hemoglobin epsilon stained by Hemoglobin epsilon antibody [HL2458] (NBP3-25500) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Hemoglobin epsilon
Alternate Names
epsilon globin, Epsilon-globin, HBEepsilon-globin, Hemoglobin epsilon chain, hemoglobin subunit epsilon, hemoglobin, epsilon 1
Gene Symbol
HBE1
Additional Hemoglobin epsilon Products
Product Documents for Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free
Product Specific Notices for Hemoglobin epsilon Antibody (HL2458) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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