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Hemoglobin epsilon Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43557

Novus Biologicals, part of Bio-Techne
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NBP2-43557

Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Primate (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Hemoglobin epsilon. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hemoglobin epsilon Antibody

Western Blot: Hemoglobin epsilon Antibody [NBP2-43557]

Western Blot: Hemoglobin epsilon Antibody [NBP2-43557]

Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Untreated (-) and treated (+) mouse tissue extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term. (CFA = Complete Freund's adjuvant)
Hemoglobin epsilon Antibody

Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] -

Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (NBP2-43557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Hemoglobin epsilon Antibody

Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] -

Western Blot: Hemoglobin epsilon Antibody [NBP2-43557] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (NBP2-43557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Hemoglobin epsilon Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000
Application Notes
IHC, IHC-P Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Hemoglobin epsilon

The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with twozeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together withtwo alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal,and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in thefollowing order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3' (provided by RefSeq)

Alternate Names

epsilon globin, Epsilon-globin, HBEepsilon-globin, Hemoglobin epsilon chain, hemoglobin subunit epsilon, hemoglobin, epsilon 1

Gene Symbol

HBE1

Additional Hemoglobin epsilon Products

Product Documents for Hemoglobin epsilon Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Hemoglobin epsilon Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.