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Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89375

Novus Biologicals, part of Bio-Techne
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NBP1-89375

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TQVFYNHMQKHKPYPSIEEFLVRDGRLNVDYKALNRSLYHVHMQNWLRFFPLRHIHIVDGDRLIRDPFPEIQKVERFLKLSPQINASNFYFNKTKGFYCLRDSGRDRCLHESKGRAHPQVDPKLLNKLHEYFHEPNKKFFELVGR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody

Western Blot: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody [NBP1-89375]

Western Blot: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody [NBP1-89375]

Western Blot: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody [NBP1-89375] - Analysis in human brain tissue.
Immunohistochemistry-Paraffin: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody [NBP1-89375]

Immunohistochemistry-Paraffin: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody [NBP1-89375]

Immunohistochemistry-Paraffin: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody [NBP1-89375] - Staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.
Western Blot: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody [NBP1-89375]

Western Blot: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody [NBP1-89375]

Western Blot: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody [NBP1-89375] - Analysis in control (vector only transfected HEK293T lysate) and HS3ST1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Applications for Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1

Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It possesses both heparan sulfate glucosaminyl 3-O-sulfotransferase activity, anticoagulant heparan sulfate conversion activity, and is a rate limiting enzyme for synthesis of anticoagulant heparan. This enzyme is an intraluminal Golgi resident protein. [provided by RefSeq]

Alternate Names

3OST1, h3-OST-1

Gene Symbol

HS3ST1

Additional Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Products

Product Documents for Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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