Hepatocyte Specific Antigen Antibody (HepPar1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45272
![Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272]](https://resources.bio-techne.com/images/products/Hepatocyte-Specific-Antigen-Antibody-HepPar1-Immunohistochemistry-Paraffin-NBP2-45272-img0002.jpg "Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Canine
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Multiplex Immunoassay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # HepPar1
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Extract of a formalin-fixed, rejected-allograft of a human liver
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 27542264).
Localization
Finely granular cytoplasmic
Marker
Hepatocellular Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08970)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Hepatocyte Specific Antigen Antibody (HepPar1)
Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272]
Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272] - Formalin-fixed, paraffin-embedded human Liver stained with Hepatocyte Specific Antigen Antibody (HepPar1).![Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272]](https://resources.bio-techne.com/images/products/Hepatocyte-Specific-Antigen-Antibody-HepPar1-Immunohistochemistry-Paraffin-NBP2-45272-img0001.jpg "Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272]")
Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272]
Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (HepPar1) [NBP2-45272] - Human Hepatocellular Carcinoma stained with HepPar-1 Monoclonal Antibody (HepPar1).Applications for Hepatocyte Specific Antigen Antibody (HepPar1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
0.25-0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Use in Western Blot reported in scientific literature (PMID:31998631) .
Optimal dilution for a specific application should be determined.
Use in Western Blot reported in scientific literature (PMID:31998631) .
Reviewed Applications
Read 1 review rated 5 using NBP2-45272 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Hepatocyte Specific Antigen
Alternate Names
Hepatocyte, HSA
Additional Hepatocyte Specific Antigen Products
Product Documents for Hepatocyte Specific Antigen Antibody (HepPar1)
Product Specific Notices for Hepatocyte Specific Antigen Antibody (HepPar1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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