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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # HSA133

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SK-H1A9-2 human hepatocellular carcinoma cells

Localization

Cell surface

Specificity

monoclonal antibody HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this monoclonal antibody on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.

Marker

Hepatocellular Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Hepatocyte Specific Antigen Antibody (HSA133) [CoraFluor™ 1]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Hepatocyte Specific Antigen

HSA (Human Serum Albumin) is useful in studying hepatocellular tumors. It may be useful in differentiating clear cell hepatocellular carcinomas from other clear cell malignancies. It has been shown in the literature to be useful in differentiating hepatoblastoma of embryonal type from small round cell tumors of childhood. It labels an antigen in the mitochondrial fraction of the liver homogenates.

Alternate Names

Hepatocyte, HSA

Additional Hepatocyte Specific Antigen Products

Product Documents for Hepatocyte Specific Antigen Antibody (HSA133) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Hepatocyte Specific Antigen Antibody (HSA133) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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