Hepatocyte Specific Antigen Antibody (SPM582) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34814
![Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (SPM582) - Azide and BSA Free [NBP2-34814]](https://resources.bio-techne.com/images/products/Hepatocyte-Specific-Antigen-Antibody-SPM582-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34814-img0001.jpg "Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (SPM582) - Azide and BSA Free [NBP2-34814]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Canine
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM582
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Extract of a formalin-fixed, rejected-allograft of a human liver
Localization
Finely granular cytoplasmic
Marker
Hepatocellular Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32859).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Hepatocyte Specific Antigen Antibody (SPM582) - Azide and BSA Free
Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (SPM582) - Azide and BSA Free [NBP2-34814]
Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (SPM582) - Azide and BSA Free [NBP2-34814] - Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with HSA Monoclonal Antibody (SPM582).Applications for Hepatocyte Specific Antigen Antibody (SPM582) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.5-1.0ug/ml
Immunohistochemistry-Frozen
1:200-1:400
Immunohistochemistry-Paraffin
0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Hepatocyte Specific Antigen
Alternate Names
Hepatocyte, HSA
Additional Hepatocyte Specific Antigen Products
Product Documents for Hepatocyte Specific Antigen Antibody (SPM582) - Azide and BSA Free
Product Specific Notices for Hepatocyte Specific Antigen Antibody (SPM582) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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