HES-1 Antibody (9I2E8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15320

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15320-100ul
NBP3-15320-20ul
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9I2E8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HES-1 Antibodies »

Product Summary for HES-1 Antibody (9I2E8)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 181-280 of human HES-1 (Q14469). FAPPPPLVPIPGGAAPPPGGAPCKLGSQAGEAAKVFGGFQVVPAPDGQFAFLIPNGAFAHSGPVIPVYTSNSGTSVGPNAVSPSSGPSLTADSMWRPWRN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HES-1 Antibody (9I2E8)

Western Blot: HES-1 Antibody (9I2E8) [NBP3-15320]

Western Blot: HES-1 Antibody (9I2E8) [NBP3-15320]

Western Blot: HES-1 Antibody (9I2E8) [NBP3-15320] - Western blot analysis of extracts of various cell lines, using HES-1 Rabbit mAb (NBP3-15320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320]

Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320]

Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320] - Immunohistochemistry of paraffin-embedded mouse spinal cord using HES-1 Rabbit mAb (NBP3-15320) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320]

Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320]

Immunohistochemistry-Paraffin: HES-1 Antibody (9I2E8) [NBP3-15320] - Immunohistochemistry of paraffin-embedded rat brain using HES-1 Rabbit mAb (NBP3-15320) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for HES-1 Antibody (9I2E8)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:100 - 1:500

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HES-1

Hes1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

Long Name

Hairy And Enhancer of Split 1

Alternate Names

HES1, HL, HRY

Gene Symbol

HES1

Additional HES-1 Products

Product Documents for HES-1 Antibody (9I2E8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HES-1 Antibody (9I2E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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