HES-1 Antibody (HL2198) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25501
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2198
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for HES-1 Antibody (HL2198) - Azide and BSA Free
Immunogen
Synthetic peptide of human HES-1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HES-1 Antibody (HL2198) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: HES-1 Antibody (HL2198) - Azide and BSA Free [NBP3-25501] -
Immunocytochemistry/Immunofluorescence: HES-1 Antibody (HL2198) - Azide and BSA Free [NBP3-25501] - HES1 antibody [HL2198] detects HES1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HES1 stained by HES1 antibody [HL2198] (NBP3-25501) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Western Blot: HES-1 Antibody (HL2198) - Azide and BSA Free [NBP3-25501] -
Western Blot: HES-1 Antibody (HL2198) - Azide and BSA Free [NBP3-25501] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [HL2198] (NBP3-25501) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Western Blot: HES-1 Antibody (HL2198) - Azide and BSA Free [NBP3-25501] -
Western Blot: HES-1 Antibody (HL2198) - Azide and BSA Free [NBP3-25501] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [HL2198] (NBP3-25501) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Applications for HES-1 Antibody (HL2198) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HES-1
Long Name
Hairy And Enhancer of Split 1
Alternate Names
HES1, HL, HRY
Gene Symbol
HES1
Additional HES-1 Products
Product Documents for HES-1 Antibody (HL2198) - Azide and BSA Free
Product Specific Notices for HES-1 Antibody (HL2198) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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