HES-1 Antibody (OTI4H1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70937

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Reverse Phase Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4H1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all HES-1 Antibodies »

Product Specifications

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 1-280 of human HES1

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HES-1 Antibody (OTI4H1) - Azide and BSA Free

Western Blot: HES-1 Antibody (OTI4H1)Azide and BSA Free [NBP2-70937]

Western Blot: HES-1 Antibody (OTI4H1)Azide and BSA Free [NBP2-70937]

Western Blot: HES-1 Antibody (OTI4H1) - Azide and BSA Free [NBP2-70937] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1 (1:500).
Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (OTI4H1) - Azide and BSA Free [NBP2-70937]

Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (OTI4H1) - Azide and BSA Free [NBP2-70937]

Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) - Azide and BSA Free [NBP2-70937] - HeLa cells using anti-HES1 mouse monoclonal antibody.
Immunohistochemistry: HES-1 Antibody (OTI4H1) - Azide and BSA Free [NBP2-70937]

Immunohistochemistry: HES-1 Antibody (OTI4H1) - Azide and BSA Free [NBP2-70937]

Immunohistochemistry: HES-1 Antibody (OTI4H1) - Azide and BSA Free [NBP2-70937] - Staining of paraffin-embedded kidney using anti-Hes1 mouse monoclonal antibody.
View 5 more images

Applications for HES-1 Antibody (OTI4H1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50RPPA 1:75

Reverse Phase Protein Array

1:75

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HES-1

Hes1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

Long Name

Hairy And Enhancer of Split 1

Alternate Names

HES1, HL, HRY

Gene Symbol

HES1

Additional HES-1 Products

Product Documents for HES-1 Antibody (OTI4H1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HES-1 Antibody (OTI4H1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.