HES-1 Antibody (SC06-21)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67642

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-67642
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SC06-21

Concentration

1 mg/ml

View all HES-1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human HES-1 aa 298-241 / 280. (SwissProt: Q14469 Human; SwissProt: P35428 Mouse; SwissProt: Q04666 Rat)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HES-1 Antibody (SC06-21)

Western Blot: HES-1 Antibody (SC06-21) [NBP2-67642]

Western Blot: HES-1 Antibody (SC06-21) [NBP2-67642]

Western Blot: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of Hes1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysate Lane 2: SK-Br-3 cell lysate
Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642]

Immunocytochemistry/ Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642]

Immunocytochemistry/Immunofluorescence: HES-1 Antibody (SC06-21) [NBP2-67642] - Staining Hes1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642]

Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642]

Immunohistochemistry-Paraffin: HES-1 Antibody (SC06-21) [NBP2-67642] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-HES-1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Applications for HES-1 Antibody (SC06-21)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:400

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HES-1

Hes1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

Long Name

Hairy And Enhancer of Split 1

Alternate Names

HES1, HL, HRY

Gene Symbol

HES1

Additional HES-1 Products

Product Documents for HES-1 Antibody (SC06-21)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HES-1 Antibody (SC06-21)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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