HES-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30912
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Predicted:
Bovine (100%), Porcine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HES-1. The exact sequence is proprietary.
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HES-1 Antibody
![Western Blot: HES-1 Antibody [NBP1-30912]](https://resources.bio-techne.com/images/products/HES-1-Antibody-Western-Blot-NBP1-30912-img0012.jpg "Western Blot: HES-1 Antibody [NBP1-30912]")
Western Blot: HES-1 Antibody [NBP1-30912]
Western Blot: HES-1 Antibody [NBP1-30912] - PC-12 whole cell extract.12 % SDS-PAGE HES1 antibody [N1], N-term dilution: 1:1000![Immunocytochemistry/ Immunofluorescence: HES-1 Antibody [NBP1-30912]](https://resources.bio-techne.com/images/products/HES-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30912-img0014.jpg "Immunocytochemistry/ Immunofluorescence: HES-1 Antibody [NBP1-30912]")
Immunocytochemistry/ Immunofluorescence: HES-1 Antibody [NBP1-30912]
Immunocytochemistry/Immunofluorescence: HES-1 Antibody [NBP1-30912] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HES1 protein stained by HES1 antibody [N1], N-term diluted at 1:2000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Hoechst 33342 staining.![Immunoprecipitation: HES-1 Antibody [NBP1-30912]](https://resources.bio-techne.com/images/products/HES-1-Antibody-Immunoprecipitation-NBP1-30912-img0011.jpg "Immunoprecipitation: HES-1 Antibody [NBP1-30912]")
Immunoprecipitation: HES-1 Antibody [NBP1-30912]
Immunoprecipitation: HES-1 Antibody [NBP1-30912] - A431 whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of HES1 by 2.5 ug of HES1 antibody 12% SDS-PAGE The immunoprecipitated HES1 protein was detected by HES1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Applications for HES-1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:2000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HES-1
Long Name
Hairy And Enhancer of Split 1
Alternate Names
HES1, HL, HRY
Gene Symbol
HES1
Additional HES-1 Products
Product Documents for HES-1 Antibody
Product Specific Notices for HES-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.