HES5 Antibody (4S4U7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16880

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16880-100ul
NBP3-16880-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4S4U7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HES-5 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 12-142 of human HES5 (Q5TA89). SPKEKNRLRKPVVEKMRRDRINSSIEQLKLLLEQEFARHQPNSKLEKADILEMAVSYLKHSKAFVAAAGPKSLHQDYSEGYSWCLQEAVQFLTLHAASDTQMKLLYHFQRPPAAPAAPAKEPKAPGAAPPP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HES5 Antibody (4S4U7)

Western Blot: HES5 Antibody (4S4U7) [NBP3-16880]

Western Blot: HES5 Antibody (4S4U7) [NBP3-16880]

Western Blot: HES5 Antibody (4S4U7) [NBP3-16880] - Analysis of extracts of various cell lines, using HES5 Rabbit mAb (NBP3-16880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: HES5 Antibody (4S4U7) [NBP3-16880]

Immunohistochemistry-Paraffin: HES5 Antibody (4S4U7) [NBP3-16880]

Immunohistochemistry-Paraffin: HES5 Antibody (4S4U7) [NBP3-16880] - Human lung cancer using HES5 Rabbit mAb (NBP3-16880) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: HES5 Antibody (4S4U7) [NBP3-16880]

Immunohistochemistry-Paraffin: HES5 Antibody (4S4U7) [NBP3-16880]

Immunohistochemistry-Paraffin: HES5 Antibody (4S4U7) [NBP3-16880] - Rat ovary using HES5 Rabbit mAb (NBP3-16880) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for HES5 Antibody (4S4U7)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HES-5

GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E.coli and purified by using conventional chromatography.

Long Name

Hairy And Enhancer of Split 5

Alternate Names

HES5

Gene Symbol

HES5

Additional HES-5 Products

Product Documents for HES5 Antibody (4S4U7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HES5 Antibody (4S4U7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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