HES5 Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41305CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP2-41305AF350

Alexa Fluor 405

NBP2-41305AF405

Alexa Fluor 488

NBP2-41305AF488

Alexa Fluor 532

NBP2-41305AF532

Alexa Fluor 594

NBP2-41305AF594

Alexa Fluor 647

NBP2-41305AF647

Alexa Fluor 700

NBP2-41305AF700

Alexa Fluor 750

NBP2-41305AF750

Biotin

NBP2-41305B

DyLight 350

NBP2-41305UV

DyLight 405

NBP2-41305V

DyLight 488

NBP2-41305G

DyLight 550

NBP2-41305R

DyLight 594

NBP2-41305DL594

DyLight 650

NBP2-41305C

DyLight 680

NBP2-41305FR

DyLight 755

NBP2-41305IR

FITC

NBP2-41305F

HRP

NBP2-41305H

Janelia Fluor 525

NBP2-41305JF525

Janelia Fluor 549

NBP2-41305JF549

Janelia Fluor 646

NBP2-41305JF646

Janelia Fluor 669

NBP2-41305JF669

mFluor Violet 450 SE

NBP2-41305MFV450

mFluor Violet 500 SE

NBP2-41305MFV500

mFluor Violet 610 SE

NBP2-41305MFV610

PerCP

NBP2-41305PCP

Forumulation

Catalog #

BSA Free

NBP2-41305

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HES-5 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5. The immunogen is located within the first 50 amino acids of HES5. Amino Acid Squence: EKNRLRKPVVEKMRRDRIN

Specificity

At least two isoforms of HES5 are known to exist; this antibody will detect both isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for HES5 Antibody [CoraFluor™ 1]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: HES-5

GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E.coli and purified by using conventional chromatography.

Long Name

Hairy And Enhancer of Split 5

Alternate Names

HES5

Gene Symbol

HES5

Additional HES-5 Products

Product Documents for HES5 Antibody [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for HES5 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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