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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human HES6 (NP_061115) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

23.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HES6 Antibody (OTI1D6) - Azide and BSA Free

Western Blot: HES6 Antibody (OTI1D6)Azide and BSA Free [NBP2-72008]

Western Blot: HES6 Antibody (OTI1D6)Azide and BSA Free [NBP2-72008]

Western Blot: HES6 Antibody (OTI1D6) - Azide and BSA Free [NBP2-72008] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES6.

Applications for HES6 Antibody (OTI1D6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HES6

HES6 is a transcription cofactor that itself does not bind to DNA but suppresses both HES1-mediated N box-dependent transcriptional repression and binding of HES1 to E box sequences. It also suppresses HES1-mediated inhibition of the heterodimer formed by ASCL1/MASH1 and TCFEA2/E47, allowing ASCL1 and TCFEA2 to upregulate transcription in its presence. HES6 appears to have novel functions in tumor and cancer cell lines, although these have not yet been elucidated fully.

Alternate Names

BHLHB41, bHLHb41Class B basic helix-loop-helix protein 41, bHLHc23, C-HAIRY1, Hairy and enhancer of split 6, hairy and enhancer of split 6 (Drosophila), HES-6, transcription cofactor HES-6

Gene Symbol

HES6

Additional HES6 Products

Product Documents for HES6 Antibody (OTI1D6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HES6 Antibody (OTI1D6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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