Hexokinase 1 Antibody (C4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-41206

Novus Biologicals, part of Bio-Techne
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NBP3-41206
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # C4

Concentration

1 mg/ml

View all Hexokinase 1 Antibodies »

Product Specifications

Immunogen

Immunized with recombinant Human Hexokinase 1 (Uniprot#: P19367; Phe80-Met215 (Uniprot#: P19367)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Hexokinase 1 Antibody (C4)

Hexokinase 1 Antibody (C4)

Western Blot: Hexokinase 1 Antibody (C4) [NBP3-41206] -

Western Blot: Hexokinase 1 Antibody (C4) [NBP3-41206] - Sample: Lane1: Rat Heart lysate; Lane2: Rat Lung lysate; Lane3: Rat Cerebrum lysate; Lane4: Jurkat cell lysate; Lane5: A549 cell lysate Primary Ab: 0.5ug/ml NBP3-41206; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Applications for Hexokinase 1 Antibody (C4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5-20ug/mL

Immunohistochemistry

5-20ug/mL

Western Blot

0.5-2ug/mL

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.01M PBS, pH7.4, 50% glycerol

Preservative

0.05% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hexokinase 1

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Alternate Names

HK1, HKI

Gene Symbol

HK1

Additional Hexokinase 1 Products

Product Documents for Hexokinase 1 Antibody (C4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Hexokinase 1 Antibody (C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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