Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Hexokinase 1 Antibodies »

Product Summary for Hexokinase 1 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 325 to 375 of human Hexokinase 1 using the numbering given in entry NP_000179.2 (GeneID 3098).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Hexokinase 1 Antibody

Western Blot: Hexokinase 1 Antibody [NBP2-32166]

Western Blot: Hexokinase 1 Antibody [NBP2-32166]

Western Blot: Hexokinase 1 Antibody [NBP2-32166] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Hexokinase antibody NBP2-32166 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Hexokinase 1 Antibody

Application
Recommended Usage

Western Blot

1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Hexokinase 1

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Alternate Names

HK1, HKI

Gene Symbol

HK1

UniProt

NP_000179.2 (Human)

Additional Hexokinase 1 Products

Product Documents for Hexokinase 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Hexokinase 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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