Hexokinase 2 Antibody (4H1)

Novus Biologicals, part of Bio-Techne | Catalog # H00003099-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat

Applications

Validated:

ELISA, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4H1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Hexokinase 2 Antibodies »

Product Specifications

Immunogen

HK2 (AAH21116, 818 a.a. ~ 917 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IVKEVCTVVARRAAQLCGAGMAAVVDRIRENRGLDALKVTVGVDGTLYKLHPHFAKVMHETVKDLAPKCDVSFLQSEDGSGKGAALITAVACRIREAGQR

Specificity

HK2 - hexokinase 2 (4H1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Hexokinase 2 Antibody (4H1)

Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01]

Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01]

Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01] - HK2 monoclonal antibody (M01), clone 4H1. Analysis of HK2 expression in A-431.
Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01]

Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01]

Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01] - HK2 monoclonal antibody (M01), clone 4H1. Analysis of HK2 expression in PC-12.
Sandwich ELISA: Hexokinase 2 Antibody (4H1) [H00003099-M01] - Detection limit for recombinant GST tagged HK2 is approximately 0.3ng/ml as a capture antibody.

Applications for Hexokinase 2 Antibody (4H1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Hexokinase 2

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Alternate Names

HK2, HKII, HXK2

Entrez Gene IDs

3099 (Human)

Gene Symbol

HK2

UniProt

P52789

Additional Hexokinase 2 Products

Product Documents for Hexokinase 2 Antibody (4H1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Hexokinase 2 Antibody (4H1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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