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HEY1 Antibody (3B3)

Novus Biologicals, part of Bio-Techne | Catalog # H00023462-M01

Novus Biologicals, part of Bio-Techne
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H00023462-M01

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

HEY1 (NP_036390, 121 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DYRSLGFRECLAEVARYLSIIEGLDASDPLRVRLVSHLNNYASQREAASGAHAGLGHIPWGTVFGHHPHIAHPLLLPQNGHGNAGTTASPTEPHHQGRLG

Specificity

hairy/enhancer-of-split related with YRPW motif 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for HEY1 Antibody (3B3)

Western Blot: HEY1 Antibody (3B3) [H00023462-M01]

Western Blot: HEY1 Antibody (3B3) [H00023462-M01]

Western Blot: HEY1 Antibody (3B3) [H00023462-M01] - analysis of HEY1 expression in transfected 293T cell line by HEY1 monoclonal antibody (M01), clone 3B3. Lane 1: HEY1 transfected lysate (32.613 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: HEY1 Antibody (3B3) [H00023462-M01]

Immunocytochemistry/ Immunofluorescence: HEY1 Antibody (3B3) [H00023462-M01]

Immunocytochemistry/Immunofluorescence: HEY1 Antibody (3B3) [H00023462-M01] - Analysis of monoclonal antibody to HEY1 on HeLa cell . Antibody concentration 10 ug/ml.
ELISA: HEY1 Antibody (3B3) [H00023462-M01]

ELISA: HEY1 Antibody (3B3) [H00023462-M01]

ELISA: HEY1 Antibody (3B3) [H00023462-M01] - Detection limit for recombinant GST tagged HEY1 is approximately 0.3ng/ml as a capture antibody.

Applications for HEY1 Antibody (3B3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HEY1

This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants.

Alternate Names

basic helix-loop-helix protein OAF1, BHLHB31, Cardiovascular helix-loop-helix factor 2, CHF-2, CHF2hHRT1, Class B basic helix-loop-helix protein 31, Hairy and enhancer of split-related protein 1, hairy/enhancer-of-split related with YRPW motif 1, hairy/enhancer-of-split related with YRPW motif protein 1, Hairy-related transcription factor 1, HERP2HES-related repressor protein 1, HESR-1, HESR1MGC1274, HES-related repressor protein 2, HRT1, HRT-1OAF1

Entrez Gene IDs

23462 (Human)

Gene Symbol

HEY1

OMIM

602953 (Human)

Additional HEY1 Products

Product Documents for HEY1 Antibody (3B3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HEY1 Antibody (3B3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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