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HGD Antibody (20D12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26234

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26234-100ul
NBP3-26234-50ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 20D12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for HGD Antibody (20D12)

Immunogen

A synthesized peptide derived from Human HGD [UniProt Q93099]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HGD Antibody (20D12)

HGD Antibody (20D12)

Immunohistochemistry: HGD Antibody (20D12) [NBP3-26234] -

Immunohistochemistry: HGD Antibody (20D12) [NBP3-26234] - Image of HGD Antibody (20D12) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
HGD Antibody (20D12)

Immunohistochemistry: HGD Antibody (20D12) [NBP3-26234] -

Immunohistochemistry: HGD Antibody (20D12) [NBP3-26234] - Image of HGD Antibody (20D12) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Applications for HGD Antibody (20D12)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: HGD

Homogentisate 1,2-dioxygenase (HGD) gene mutations are the molecular cause of alkaptonuria, a rare hereditary disorder of the phenylalanine catabolism. The highest expression of HGD is in the prostate, small intestine, colon, and liver. The HGD gene contains 14 exons. Conflicting reports have placed the gene at 3q2, 3q13.3-q21, 3q21-q24, 3q21-q23, or 3q25-q26.

Alternate Names

AKU, EC 1.13.11.5, HGOFLJ94126, homogentisate 1,2-dioxygenase, homogentisate 1,2-dioxygenase (homogentisate oxidase), homogentisate oxidase, Homogentisate oxygenase, Homogentisic acid oxidase, Homogentisicase

Gene Symbol

HGD

Additional HGD Products

Product Documents for HGD Antibody (20D12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HGD Antibody (20D12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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