HGS Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-55249
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 727 and the C-terminus (resude 777) of human growth factor-regulated tyrosine kinase substrate using the numbering given in entry NP_004703.1 (GeneID 9146).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HGS Antibody
![Western Blot: HGS Antibody [NB100-55249]](https://resources.bio-techne.com/images/products/HGS-Antibody-Western-Blot-NB100-55249-img0002.jpg "Western Blot: HGS Antibody [NB100-55249]")
Western Blot: HGS Antibody [NB100-55249]
Western Blot: HGS Antibody [NB100-55249] - Detection of Human HRS by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody NB100-55249 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.![Western Blot: HGS Antibody [NB100-55249]](https://resources.bio-techne.com/images/products/HGS-Antibody-Western-Blot-NB100-55249-img0003.jpg "Western Blot: HGS Antibody [NB100-55249]")
Western Blot: HGS Antibody [NB100-55249]
Western Blot: HGS Antibody [NB100-55249] - Whole cell lysate (15 ug) from Renca, CT26.WT and 4T1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HRS antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: HGS Antibody [NB100-55249]](https://resources.bio-techne.com/images/products/HGS-Antibody-Immunoprecipitation-NB100-55249-img0004.jpg "Immunoprecipitation: HGS Antibody [NB100-55249]")
Immunoprecipitation: HGS Antibody [NB100-55249]
Immunoprecipitation: HGS Antibody [NB100-55249] - Detection of human HRS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HRS antibody NB100-55249 (lot NB100-55249-2) used for IP at 6 ug per reaction. HRS was also immunoprecipitated by a previous lot of this antibody (NB100-55249-1) and another rabbit anti-HRS antibody. For blotting immunoprecipitated HRS, NB100-55249 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Applications for HGS Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HGS
Alternate Names
FLJ39878, hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, HRSFLJ11421, human growth factor-regulated tyrosine kinase substrate, Protein pp110, Vps27, ZFYVE8
Entrez Gene IDs
9146 (Human)
Gene Symbol
HGS
UniProt
Additional HGS Products
Product Documents for HGS Antibody
Product Specific Notices for HGS Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...