hHpr1-p84-Thoc1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47304
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 607 and 657 of human THO Complex 1 using the numbering given in entry NP_005122.2 (GeneID 9984).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for hHpr1-p84-Thoc1 Antibody
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-47304]](https://resources.bio-techne.com/images/products/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP1-47304-img0005.jpg "Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-47304]")
Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-47304]
Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - Detection of Human THOC1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THOC1 antibody NBP1-47304 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-47304]](https://resources.bio-techne.com/images/products/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-NBP1-47304-img0003.jpg "Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-47304]")
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-47304]
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- THOC1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate![Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP1-47304]](https://resources.bio-techne.com/images/products/hHpr1-p84-Thoc1-Antibody-Immunoprecipitation-NBP1-47304-img0006.jpg "Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP1-47304]")
Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP1-47304]
Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - Detection of human THOC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-THOC1 antibody NBP1-47304 used for IP at 3 ug per reaction. THOC1 was also immunoprecipitated by another rabbit anti-THOC1 antibody. For blotting immunoprecipitated THOC1, NBP1-47304 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Applications for hHpr1-p84-Thoc1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: hHpr1-p84-Thoc1
Alternate Names
HPR1THO complex subunit 1, hTREX84, Nuclear matrix protein p84, P84, p84N5, THO complex 1, tho1
Entrez Gene IDs
9984 (Human)
Gene Symbol
THOC1
Additional hHpr1-p84-Thoc1 Products
Product Documents for hHpr1-p84-Thoc1 Antibody
Product Specific Notices for hHpr1-p84-Thoc1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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