hHpr1-p84-Thoc1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89670

Novus Biologicals, part of Bio-Techne
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NBP1-89670
NBP1-89670-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all hHpr1-p84-Thoc1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KILMGNEELTRLWNLCPDNMEACKSETREHMPTLEEFFEEAIEQADPENMVENEYKAVNNSNYGWRALRLLARRSPHFFQPTNQQFKSLPEYLENMVIKLAKELPPPSEEIKTGEDEDEEDNDALLK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for hHpr1-p84-Thoc1 Antibody

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670]

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670]

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining in human testis and skeletal muscle tissues using anti-THOC1 antibody. Corresponding THOC1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670]

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670]

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human cerebral cortex, colon, skeletal muscle and testis using Anti-THOC1 antibody NBP1-89670 (A) shows similar protein distribution across tissues to independent antibody NBP1-89669 (B).
Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-89670]

Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-89670]

Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Analysis using Anti-THOC1 antibody NBP1-89670 (A) shows similar pattern to independent antibody NBP1-89669 (B).
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Applications for hHpr1-p84-Thoc1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: hHpr1-p84-Thoc1

P84/N5-5E10 recognizes an 84kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.

Alternate Names

HPR1THO complex subunit 1, hTREX84, Nuclear matrix protein p84, P84, p84N5, THO complex 1, tho1

Gene Symbol

THOC1

Additional hHpr1-p84-Thoc1 Products

Product Documents for hHpr1-p84-Thoc1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for hHpr1-p84-Thoc1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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