hHpr1-p84-Thoc1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38514
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 428-657 of human hHpr1-p84-Thoc1 (hHpr1-p84-Thoc1 (THOC1)) (NP_005122.2).
Sequence:
TKKILMGNEELTRLWNLCPDNMEACKSETREHMPTLEEFFEEAIEQADPENMVENEYKAVNNSNYGWRALRLLARRSPHFFQPTNQQFKSLPEYLENMVIKLAKELPPPSEEIKTGEDEDEEDNDALLKENESPDVRRDKPVTGEQIEVFANKLGEQWKILAPYLEMKDSEIRQIECDSEDMKMRAKQLLVAWQDQEGVHATPENLINALNKSGLSDLAESLTNDNETNS
Sequence:
TKKILMGNEELTRLWNLCPDNMEACKSETREHMPTLEEFFEEAIEQADPENMVENEYKAVNNSNYGWRALRLLARRSPHFFQPTNQQFKSLPEYLENMVIKLAKELPPPSEEIKTGEDEDEEDNDALLKENESPDVRRDKPVTGEQIEVFANKLGEQWKILAPYLEMKDSEIRQIECDSEDMKMRAKQLLVAWQDQEGVHATPENLINALNKSGLSDLAESLTNDNETNS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for hHpr1-p84-Thoc1 Antibody
Western Blot: hHpr1-p84-Thoc1 Antibody [NBP3-38514] -
Western Blot: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Western blot analysis of various lysates using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: hHpr1-p84-Thoc1 Antibody [NBP3-38514] -
Immunocytochemistry/ Immunofluorescence: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Immunofluorescence analysis of U2OS cells using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunohistochemistry: hHpr1-p84-Thoc1 Antibody [NBP3-38514] -
Immunohistochemistry: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Applications for hHpr1-p84-Thoc1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:100
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: hHpr1-p84-Thoc1
Alternate Names
HPR1THO complex subunit 1, hTREX84, Nuclear matrix protein p84, P84, p84N5, THO complex 1, tho1
Gene Symbol
THOC1
Additional hHpr1-p84-Thoc1 Products
Product Specific Notices for hHpr1-p84-Thoc1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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