hHR23b Antibody (3H7)

Novus Biologicals, part of Bio-Techne | Catalog # H00005887-M10

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3H7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all hHR23b Antibodies »

Product Specifications

Immunogen

RAD23B (NP_002865.1, 311 a.a. ~ 409 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PQLLQQISQHQEHFIQMLNEPVQEAGGQGGGGGGGSGGIAEAGSGHMNYIQVTPQEKEAIERLKALGFPEGLVIQAYFACEKNENLAANFLLQQNFDED

Specificity

RAD23B - RAD23 homolog B (S. cerevisiae) (3H7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for hHR23b Antibody (3H7)

Western Blot: hHR23b Antibody (3H7) [H00005887-M10]

Western Blot: hHR23b Antibody (3H7) [H00005887-M10]

Western Blot: hHR23b Antibody (3H7) [H00005887-M10] - Analysis of RAD23B expression in transfected 293T cell line by RAD23B monoclonal antibody (M10), clone 3H7. Lane 1: RAD23B transfected lysatE (43.2 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: hHR23b Antibody (3H7) [H00005887-M10]

Immunohistochemistry-Paraffin: hHR23b Antibody (3H7) [H00005887-M10]

Immunohistochemistry-Paraffin: hHR23b Antibody (3H7) [H00005887-M10] - Analysis of monoclonal antibody to RAD23B on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml
Sandwich ELISA: hHR23b Antibody (3H7) [H00005887-M10] - Detection limit for recombinant GST tagged RAD23B is 0.1 ng/ml as a capture antibody.

Applications for hHR23b Antibody (3H7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: hHR23b

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. [provided by RefSeq]

Alternate Names

HHR23B, HR23BUV excision repair protein RAD23 homolog B, P58, RAD23 (S. cerevisiae) homolog B, RAD23 homolog B (S. cerevisiae), RAD23, yeast homolog of, B, XP-C repair complementing complex 58 kDa, XP-C repair complementing protein, XP-C repair-complementing complex 58 kDa protein

Entrez Gene IDs

5887 (Human)

Gene Symbol

RAD23B

UniProt

NP_002865 (Human)

Additional hHR23b Products

Product Documents for hHR23b Antibody (3H7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for hHR23b Antibody (3H7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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