HIBCH Antibody (OTI3H5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71796

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Canine

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3H5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all HIBCH Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HIBCH (NP_055177) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

39.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HIBCH Antibody (OTI3H5) - Azide and BSA Free

Western Blot: HIBCH Antibody (OTI3H5)Azide and BSA Free [NBP2-71796]

Western Blot: HIBCH Antibody (OTI3H5)Azide and BSA Free [NBP2-71796]

Western Blot: HIBCH Antibody (OTI3H5) - Azide and BSA Free [NBP2-71796] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH.
Immunohistochemistry: HIBCH Antibody (OTI3H5) - Azide and BSA Free [NBP2-71796]

Immunohistochemistry: HIBCH Antibody (OTI3H5) - Azide and BSA Free [NBP2-71796]

Immunohistochemistry: HIBCH Antibody (OTI3H5) - Azide and BSA Free [NBP2-71796] - Staining of paraffin-embedded Human Kidney tissue using anti-HIBCH mouse monoclonal antibody.
Western Blot: HIBCH Antibody (OTI3H5)Azide and BSA Free [NBP2-71796]

Western Blot: HIBCH Antibody (OTI3H5)Azide and BSA Free [NBP2-71796]

Western Blot: HIBCH Antibody (OTI3H5) - Azide and BSA Free [NBP2-71796] - Analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.
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Applications for HIBCH Antibody (OTI3H5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:50

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HIBCH

Beta-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) is responsible for the specific hydrolysis of HIBYL-CoA, a valine catabolite, as well as the hydrolysis of beta-hydroxypropionyl-CoA, an intermediate in a minor pathway of propionate metabolism.(supplied by OMIM)

Alternate Names

3-hydroxyisobutyryl-CoA hydrolase, EC 3.1.2.4,3-hydroxyisobutyryl-coenzyme A hydrolase3-hydroxyisobutyryl-CoA hydrolase, mitochondrial, HIB-CoA hydrolase, HIBYLCOAH, HIBYL-CoA-H

Gene Symbol

HIBCH

Additional HIBCH Products

Product Documents for HIBCH Antibody (OTI3H5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HIBCH Antibody (OTI3H5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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