HIBCH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32669

Novus Biologicals, part of Bio-Techne
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NBP2-32669
NBP2-32669-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HIBCH Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACMRGHDFHEGVRAVLIDKDQSPKWKPADLKEVTEEDLNNHFKSLGSSDL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HIBCH Antibody

Western Blot: HIBCH Antibody [NBP2-32669]

Western Blot: HIBCH Antibody [NBP2-32669]

Western Blot: HIBCH Antibody [NBP2-32669] - Analysis using Anti-HIBCH antibody NBP2-32669 (A) shows similar pattern to independent antibody NBP1-85891 (B).
Immunocytochemistry/ Immunofluorescence: HIBCH Antibody [NBP2-32669]

Immunocytochemistry/ Immunofluorescence: HIBCH Antibody [NBP2-32669]

Immunocytochemistry/Immunofluorescence: HIBCH Antibody [NBP2-32669] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: HIBCH Antibody [NBP2-32669]

Immunohistochemistry-Paraffin: HIBCH Antibody [NBP2-32669]

Immunohistochemistry-Paraffin: HIBCH Antibody [NBP2-32669] - Staining of human liver.
View 6 more images

Applications for HIBCH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HIBCH

Beta-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) is responsible for the specific hydrolysis of HIBYL-CoA, a valine catabolite, as well as the hydrolysis of beta-hydroxypropionyl-CoA, an intermediate in a minor pathway of propionate metabolism.(supplied by OMIM)

Alternate Names

3-hydroxyisobutyryl-CoA hydrolase, EC 3.1.2.4,3-hydroxyisobutyryl-coenzyme A hydrolase3-hydroxyisobutyryl-CoA hydrolase, mitochondrial, HIB-CoA hydrolase, HIBYLCOAH, HIBYL-CoA-H

Gene Symbol

HIBCH

Additional HIBCH Products

Product Documents for HIBCH Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HIBCH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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