HIC2 Antibody (PCRP-HIC2-2F8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13891
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # PCRP-HIC2-2F8
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human HIC2protein (Uniprot: Q96JB3)
Localization
Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
66.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14235)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Applications for HIC2 Antibody (PCRP-HIC2-2F8)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunoprecipitation
1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: HIC2
Alternate Names
HIC1-related gene on chromosome 22 protein, Hic-2, Hic-3, HRG22KIAA1020hic-2, hypermethylated in cancer 2, hypermethylated in cancer 2 protein, ZBTB30hic-3, Zinc finger and BTB domain-containing protein 30, ZNF907
Gene Symbol
HIC2
UniProt
Additional HIC2 Products
Product Documents for HIC2 Antibody (PCRP-HIC2-2F8)
Product Specific Notices for HIC2 Antibody (PCRP-HIC2-2F8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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