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HINT2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86024

Novus Biologicals, part of Bio-Techne
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NBP1-86024
NBP1-86024-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IPRISQAEEEDQQLLGHLLLVAKQTAKAEGLGDGYRLVINDGKLGAQSVYHLHIHVLGGRQLQWP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HINT2 Antibody

Immunohistochemistry-Paraffin: HINT2 Antibody [NBP1-86024]

Immunohistochemistry-Paraffin: HINT2 Antibody [NBP1-86024]

Immunohistochemistry-Paraffin: HINT2 Antibody [NBP1-86024] - Staining of human fallopian tube, gastrointestinal, kidney and testis using Anti-HINT2 antibody NBP1-86024 (A) shows similar protein distribution across tissues to independent antibody NBP2-56156 (B).
Western Blot: HINT2 Antibody [NBP1-86024]

Western Blot: HINT2 Antibody [NBP1-86024]

Western Blot: HINT2 Antibody [NBP1-86024] - Analysis in human cell line PC-3.
Immunocytochemistry/ Immunofluorescence: HINT2 Antibody [NBP1-86024]

Immunocytochemistry/ Immunofluorescence: HINT2 Antibody [NBP1-86024]

Immunocytochemistry/Immunofluorescence: HINT2 Antibody [NBP1-86024] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.

Applications for HINT2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HINT2

Histidine triad proteins, such as HINT2, are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides (Brenner, 2002 [PubMed 12119013]).[supplied by OMIM]

Alternate Names

EC 3.-, HINT-2, HINT-3, histidine triad nucleotide binding protein 2, histidine triad nucleotide-binding protein 2, mitochondrial, HIT-17, HIT-17kDa, PKCI-1-related HIT protein, protein kinase C inhibitor-2

Gene Symbol

HINT2

Additional HINT2 Products

Product Documents for HINT2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HINT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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