HIPK3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24521

Novus Biologicals, part of Bio-Techne
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NBP2-24521
NBP2-24521SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Primate

Predicted:

Chimpanzee (100%), Monkey (94%), Orangutan (94%), Rhesus Macaque (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all HIPK3 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 850-900 of human HIPK3 was used as the immunogen.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Equine (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HIPK3 Antibody - BSA Free

Western Blot: HIPK3 AntibodyBSA Free [NBP2-24521]

Western Blot: HIPK3 AntibodyBSA Free [NBP2-24521]

Western Blot: HIPK3 Antibody [NBP2-24521] - Analysis of HIPK3 in mouse testis lysate in the 1) absence, 2) presence of immunizing peptide and 3) rat testis using this antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/ Immunofluorescence: HIPK3 Antibody - BSA Free [NBP2-24521]

Immunocytochemistry/ Immunofluorescence: HIPK3 Antibody - BSA Free [NBP2-24521]

Immunocytochemistry/Immunofluorescence: HIPK3 Antibody [NBP2-24521] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-HIPK3 [NBP2-24521] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: HIPK3 Antibody - BSA Free [NBP2-24521]

Immunohistochemistry-Paraffin: HIPK3 Antibody - BSA Free [NBP2-24521]

Immunohistochemistry-Paraffin: HIPK3 Antibody [NBP2-24521] - Analysis of human testis using this antibody at 10 ug/ml.
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Applications for HIPK3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry-Paraffin

5-10 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HIPK3

Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors

Alternate Names

Androgen receptor-interacting nuclear protein kinase, DYRK6Fas-interacting serine/threonine-protein kinase, EC 2.7.11.1, FIST, FIST3ANPK, homeodomain interacting protein kinase 3, homeodomain-interacting protein kinase 3, PKYHomolog of protein kinase YAK1, YAK1

Gene Symbol

HIPK3

UniProt

Q9H422 (Human)

Additional HIPK3 Products

Product Documents for HIPK3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HIPK3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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