Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all HISPPD1 Antibodies »

Product Summary for HISPPD1 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 925 to 975 of human Diphosphoinositol Pentakisphosphate Kinase 2 using the numbering given in entry BAA24863.2 (GeneID 23262).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HISPPD1 Antibody

Western Blot: HISPPD1 Antibody [NBP2-32155]

Western Blot: HISPPD1 Antibody [NBP2-32155]

Western Blot: HISPPD1 Antibody [NBP2-32155] - Samples: Whole cell lysate (50 ug) from K562, HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-HISPPD1 antibody NBP2-32155 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: HISPPD1 Antibody [NBP2-32155]

Immunoprecipitation: HISPPD1 Antibody [NBP2-32155]

Immunoprecipitation: HISPPD1 Antibody [NBP2-32155] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HISPPD1 antibody NBP2-32155 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated HISPPD1, NBP2-32155 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for HISPPD1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HISPPD1

Inositol phosphates (IPs) and diphosphoinositol phosphates (PP-IPs), also known as inositol pyrophosphates, act as cell signaling molecules. HISPPD1 has both IP6 kinase (EC 2.7.4.21) and PP-IP5 (also called IP7) kinase (EC 2.7.4.24) activities that produce the high-energy pyrophosphates PP-IP5 and PP2-IP4 (also called IP8), respectively (Fridy et al., 2007 (PubMed 17690096)).(supplied by OMIM)

Alternate Names

diphosphoinositol pentakisphosphate kinase 2FLJ21506, EC 2.7.4.21, EC 2.7.4.24, HISPPD1histidine acid phosphatase domain containing 1, Histidine acid phosphatase domain-containing protein 1, hsVIP2, inositol heptaphosphate kinase 2, inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2, InsP6 and PP-IP5 kinase 2, KIAA0433FLJ23463, VIP1 homolog 2, VIP2IP7K2

Gene Symbol

PPIP5K2

UniProt

BAA24863.2 (Human)

Additional HISPPD1 Products

Product Documents for HISPPD1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HISPPD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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