HIST1H2BK Antibody (4G10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26229
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4G10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HIST1H2BK Antibody (4G10)
Immunogen
Synthesized peptide [UniProt O60814]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HIST1H2BK Antibody (4G10)
Immunohistochemistry: HIST1H2BK Antibody (4G10) [NBP3-26229] -
Immunohistochemistry: HIST1H2BK Antibody (4G10) [NBP3-26229] - Image of HIST1H2BK Antibody (4G10) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot: HIST1H2BK Antibody (4G10) [NBP3-26229] -
Western Blot: HIST1H2BK Antibody (4G10) [NBP3-26229] - Positive Western Blot detected in: Mouse brain tissue, Rat liver tissue, Rat lung tissue.All lanes: HIST1H2BK Antibody at 0.16ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 14 KDa
Observed band size: 14 KDa
Applications for HIST1H2BK Antibody (4G10)
Application
Recommended Usage
Immunohistochemistry
1:50-1:500
Western Blot
1:5000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: HIST1H2BK
Alternate Names
H2B histone family, member T, H2B K, H2B/S, H2BFAiii, H2BFT, H2bk, HIRA-interacting protein 1, HIRIP1, histone cluster 1, H2bk, histone family member, histone H2B type 1-K, MGC131989
Gene Symbol
H2BC12
Additional HIST1H2BK Products
Product Documents for HIST1H2BK Antibody (4G10)
Product Specific Notices for HIST1H2BK Antibody (4G10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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