HIST1H3A Antibody (CL4967)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61150

Novus Biologicals, part of Bio-Techne
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NBP2-61150
NBP2-61150-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL4967

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HIST1H3A Antibodies »

Product Summary for HIST1H3A Antibody (CL4967)

Immunogen

This antibody was developed against a synthetic peptide corresponding to the following amino acid sequence:PKDIQLARRIRGERA

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for HIST1H3A Antibody (CL4967)

Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150]

Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150]

Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis of purified human recombinant histone H3, CenH3, H3.3, H2A, H2B and H4.
Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (CL4967) [NBP2-61150]

Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (CL4967) [NBP2-61150]

Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (CL4967) [NBP2-61150] - Staining of U-2 OS cells using the Anti-HIST1H3A monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150]

Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150]

Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human kidney shows strong nuclear positivity in renal tubules cells.
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Applications for HIST1H3A Antibody (CL4967)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:10000 - 1:20000

Immunohistochemistry-Paraffin

1:10000 - 1:20000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HIST1H3A

Alternate Names

H3 histone family, member A, H3/A, H3FAHIST1H3E, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, histone cluster 1, H3a, histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

Gene Symbol

H3C1

Additional HIST1H3A Products

Product Documents for HIST1H3A Antibody (CL4967)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HIST1H3A Antibody (CL4967)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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