HIST1H3A Antibody (JU43-26)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75522

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JU43-26

Concentration

1 mg/ml

View all HIST1H3A Antibodies »

Product Summary for HIST1H3A Antibody (JU43-26)

Immunogen

Synthetic peptide within Human HIST1H3A aa 1-50 / 136 (acetyl K14) . (SwissProt: P68431 Human; SwissProt: P68433 Mouse; SwissProt: Q6LED0 Rat)

Localization

Nucleus. Chromosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HIST1H3A Antibody (JU43-26)

Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]

Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]

Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Western blot analysis of HIST1H3A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: rat liver tissue lysate Lane 3: mouse lung tissue lysate
Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]

Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]

Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Staining Histone H3 (acetyl K14) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522]

Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522]

Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of paraffin-embedded rat brain tissue using anti-Histone H3 (acetyl K14) antibody. Counter stained with hematoxylin.
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Applications for HIST1H3A Antibody (JU43-26)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HIST1H3A

Alternate Names

H3 histone family, member A, H3/A, H3FAHIST1H3E, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, histone cluster 1, H3a, histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

Gene Symbol

H3C1

Additional HIST1H3A Products

Product Documents for HIST1H3A Antibody (JU43-26)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HIST1H3A Antibody (JU43-26)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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