HIST1H3A Antibody (JU43-26)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75522
Recombinant Monoclonal Antibody.
![Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-JU43-26-Western-Blot-NBP2-75522-img0006.jpg "Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Chromatin Immunoprecipitation, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JU43-26
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human HIST1H3A aa 1-50 / 136 (acetyl K14) . (SwissProt: P68431 Human; SwissProt: P68433 Mouse; SwissProt: Q6LED0 Rat)
Localization
Nucleus. Chromosome.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HIST1H3A Antibody (JU43-26)
![Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-JU43-26-Western-Blot-NBP2-75522-img0008.jpg "Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]
Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Western blot analysis of HIST1H3A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: rat liver tissue lysate Lane 3: mouse lung tissue lysate![Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-JU43-26-Immunocytochemistry-Immunofluorescence-NBP2-75522-img0003.jpg "Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]
Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Staining Histone H3 (acetyl K14) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-JU43-26-Immunohistochemistry-Paraffin-NBP2-75522-img0005.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522]
Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of paraffin-embedded rat brain tissue using anti-Histone H3 (acetyl K14) antibody. Counter stained with hematoxylin.![Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-JU43-26-Western-Blot-NBP2-75522-img0007.jpg "Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
![Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-JU43-26-Immunocytochemistry-Immunofluorescence-NBP2-75522-img0001.jpg "Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
![Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-JU43-26-Immunocytochemistry-Immunofluorescence-NBP2-75522-img0002.jpg "Immunocytochemistry/ Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522]](https://resources.bio-techne.com/images/products/HIST1H3A-Antibody-JU43-26-Immunohistochemistry-Paraffin-NBP2-75522-img0004.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522]")
Applications for HIST1H3A Antibody (JU43-26)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HIST1H3A
Alternate Names
H3 histone family, member A, H3/A, H3FAHIST1H3E, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone 1, H3a, histone cluster 1, H3a, histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Gene Symbol
H3C1
Additional HIST1H3A Products
Product Documents for HIST1H3A Antibody (JU43-26)
Product Specific Notices for HIST1H3A Antibody (JU43-26)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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