Histamine H2R Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-812

Novus Biologicals, part of Bio-Techne
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NB600-812
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Histamine H2 R Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QEEKPLKLQVWSGTE corresponding to C-Terminus according to NP_071640.1, NP_001124527.1.

Specificity

This antibody is expected to recognise both reported isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Histamine H2R Antibody

Western Blot: Histamine H2R Antibody [NB600-812]

Western Blot: Histamine H2R Antibody [NB600-812]

Western Blot: Histamine H2R Antibody [NB600-812] - Staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Histamine H2R Antibody [NB600-812]

Immunocytochemistry/ Immunofluorescence: Histamine H2R Antibody [NB600-812]

Immunocytochemistry/Immunofluorescence: Histamine H2R Antibody [NB600-812] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: Histamine H2R Antibody [NB600-812]

Flow Cytometry: Histamine H2R Antibody [NB600-812]

Flow Cytometry: Histamine H2R Antibody [NB600-812] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for Histamine H2R Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.5 - 2 ug/mL
Application Notes
Western blot: Approx 50kDa band observed in Human Tonsil lysates (calculated MW of 46.9kDa according to NP_001354640.1). Recommended concentration: 0.5-2ug/ml. Primary incubation 1 hour at room temperature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histamine H2 R

This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.

Long Name

Histamine H2 Receptor

Alternate Names

Gastric Receptor 1, Histamine H2R, HRH2

Entrez Gene IDs

3274 (Human)

Gene Symbol

HRH2

UniProt

NP_071640.1 (Human)

Additional Histamine H2 R Products

Product Documents for Histamine H2R Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histamine H2R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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