Histamine H3R Antibody (7H8E6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16202
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7H8E6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histamine H3R (Q9Y5N1). MERAPPDGPLNASGALAGEAAAAGGARGFSAAWTAVLAALMALLIVATVLGNALVMLAFVADSSLRTQNNFFLLNLAISDFLVGAFCIPLYVPYVLTGRW
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Histamine H3R Antibody (7H8E6)
Western Blot: Histamine H3R Antibody (7H8E6) [NBP3-16202] -
Western Blot: Histamine H3R Antibody (7H8E6) [NBP3-16202] - Western blot analysis of various lysates using Histamine H3R Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.
Immunoprecipitation: Histamine H3R Antibody (7H8E6) [NBP3-16202] -
Immunoprecipitation: Histamine H3R Antibody (7H8E6) [NBP3-16202] - Immunoprecipitation analysis of 300 ug extracts of Raji cells using 3 ug Histamine H3R antibody. Western blot was performed from the immunoprecipitate using Histamine H3R antibody at a dilution of 1:1000.
Western Blot: Histamine H3R Antibody (7H8E6) [NBP3-16202] -
Western Blot: Histamine H3R Antibody (7H8E6) [NBP3-16202] - Western blot analysis of various lysates using Histamine H3R Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Applications for Histamine H3R Antibody (7H8E6)
Application
Recommended Usage
Immunoprecipitation
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histamine H3 R
Long Name
Histamine H3 Receptor
Alternate Names
GPCR97, HH3R, Histamine H3R, HRH3
Gene Symbol
HRH3
Additional Histamine H3 R Products
Product Documents for Histamine H3R Antibody (7H8E6)
Product Specific Notices for Histamine H3R Antibody (7H8E6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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