Histamine N-Methyltransferase/HNMT Antibody (034)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90171

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 034

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histamine N-Methyltransferase/HNMT Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Histamine N-Methyltransferase/HNMT (Accession#: AAH20677.1; Met1-Ala292).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Histamine N-Methyltransferase/HNMT Antibody (034)

Western Blot: Histamine N-Methyltransferase/HNMT Antibody (034) [NBP2-90171]

Western Blot: Histamine N-Methyltransferase/HNMT Antibody (034) [NBP2-90171]

Western Blot: Histamine N-Methyltransferase/HNMT Antibody (034) [NBP2-90171] - Anti-HNMT rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Predicted band size:33 kDa. Observed band size:33 kDa.
Immunoprecipitation: Histamine N-Methyltransferase/HNMT Antibody (034) [NBP2-90171]

Immunoprecipitation: Histamine N-Methyltransferase/HNMT Antibody (034) [NBP2-90171]

Immunoprecipitation: Histamine N-Methyltransferase/HNMT Antibody (034) [NBP2-90171] - HNMT was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate. Lane B:0.5 mg Hela Whole Cell Lysate. 2 uL anti-HNMT rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-HNMT rabbit monoclonal antibody,at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 33 kDa.
Immunoprecipitation: Histamine N-Methyltransferase/HNMT Antibody (034) [NBP2-90171]

Immunoprecipitation: Histamine N-Methyltransferase/HNMT Antibody (034) [NBP2-90171]

Immunoprecipitation: Histamine N-Methyltransferase/HNMT Antibody (034) [NBP2-90171] - HNMT was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate. Lane B:0.5 mg Hela Whole Cell Lysate. 2 uL anti-HNMT rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-HNMT rabbit monoclonal antibody,at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 33 kDa.

Applications for Histamine N-Methyltransferase/HNMT Antibody (034)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histamine N-Methyltransferase/HNMT

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.

Long Name

Histamine N-Methyltransferase

Alternate Names

Histamine NMethyltransferase, HMT, HNMT, HNMT-S1, HNMT-S2

Gene Symbol

HNMT

Additional Histamine N-Methyltransferase/HNMT Products

Product Documents for Histamine N-Methyltransferase/HNMT Antibody (034)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histamine N-Methyltransferase/HNMT Antibody (034)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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