Histamine N-Methyltransferase/HNMT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38253

Novus Biologicals, part of Bio-Techne
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NBP2-38253
NBP2-38253-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Histamine N-Methyltransferase/HNMT Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TSDDLTQMLDNLGLKYECYDLLSTMDISDCFIDGNENGDLLWDFLTETCNFNATAPPDLRAELGKDLQEPEFSAKKEGKVLFNNTLSFIVIEA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histamine N-Methyltransferase/HNMT Antibody

Western Blot: Histamine N-Methyltransferase/HNMT Antibody [NBP2-38253]

Western Blot: Histamine N-Methyltransferase/HNMT Antibody [NBP2-38253]

Western Blot: Histamine N-Methyltransferase/HNMT Antibody [NBP2-38253] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP2-38253]

Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP2-38253]

Immunohistochemistry-Paraffin: Histamine N-Methyltransferase/HNMT Antibody [NBP2-38253] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Applications for Histamine N-Methyltransferase/HNMT Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histamine N-Methyltransferase/HNMT

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.

Long Name

Histamine N-Methyltransferase

Alternate Names

Histamine NMethyltransferase, HMT, HNMT, HNMT-S1, HNMT-S2

Gene Symbol

HNMT

UniProt

P50135 (Human)

Additional Histamine N-Methyltransferase/HNMT Products

Product Documents for Histamine N-Methyltransferase/HNMT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Histamine N-Methyltransferase/HNMT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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