Histidase Antibody (4F2)
Novus Biologicals, part of Bio-Techne | Catalog # H00003034-M04
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4F2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
HAL (NP_002099, 558 a.a. ~ 657 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LAACQGIEFLRPLKTTTPLEKVYDLVRSVVRPWIKDRFMAPDIEAAHRLLLEQKVWEVAAPYIEKYRMEHIPESRPLSPTAFSLQFLHKKSTKIPESEDL
Specificity
HAL (4F2)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Histidase Antibody (4F2)
![Western Blot: Histidase Antibody (4F2) [H00003034-M04]](https://resources.bio-techne.com/images/products/Histidase-Antibody-4F2-Western-Blot-H00003034-M04-img0003.jpg "Western Blot: Histidase Antibody (4F2) [H00003034-M04]")
Western Blot: Histidase Antibody (4F2) [H00003034-M04]
Western Blot: Histidase Antibody (4F2) [H00003034-M04] - Analysis of HAL expression in transfected 293T cell line by HAL monoclonal antibody (M04), clone 4F2. Lane 1: HAL transfected lysatE (72.698 KDa). Lane 2: Non-transfected lysate.![Immunoprecipitation: Histidase Antibody (4F2) [H00003034-M04]](https://resources.bio-techne.com/images/products/Histidase-Antibody-4F2-Immunoprecipitation-H00003034-M04-img0002.jpg "Immunoprecipitation: Histidase Antibody (4F2) [H00003034-M04]")
Immunoprecipitation: Histidase Antibody (4F2) [H00003034-M04]
Immunoprecipitation: Histidase Antibody (4F2) [H00003034-M04] - Analysis of HAL transfected lysate using anti-HAL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HAL rabbit polyclonal antibody.Applications for Histidase Antibody (4F2)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Histidase
Alternate Names
EC 4.3.1, EC 4.3.1.3, HIS, Histidase, histidine ammonia-lyase, HSTD
Entrez Gene IDs
3034 (Human)
Gene Symbol
HAL
OMIM
235800 (Human)
UniProt
Additional Histidase Products
Product Documents for Histidase Antibody (4F2)
Product Specific Notices for Histidase Antibody (4F2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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