Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7E10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Histone Deacetylase 2/HDAC2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 415-488 of human HDAC2(NP_001518) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

55.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone Deacetylase 2/HDAC2 Antibody (OTI7E10) - Azide and BSA Free

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (OTI7E10)Azide and BSA Free [NBP2-70881]

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (OTI7E10)Azide and BSA Free [NBP2-70881]

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (OTI7E10) - Azide and BSA Free [NBP2-70881] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC2.
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (OTI7E10) - Azide and BSA Free [NBP2-70881]

Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (OTI7E10) - Azide and BSA Free [NBP2-70881]

Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (OTI7E10) - Azide and BSA Free [NBP2-70881] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (OTI7E10) - Azide and BSA Free [NBP2-70881]

Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (OTI7E10) - Azide and BSA Free [NBP2-70881]

Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (OTI7E10) - Azide and BSA Free [NBP2-70881] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
View 15 more images

Applications for Histone Deacetylase 2/HDAC2 Antibody (OTI7E10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 2/HDAC2

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

Long Name

Histone Deacetylase 2

Alternate Names

HDAC2, YAF1

Gene Symbol

HDAC2

Additional Histone Deacetylase 2/HDAC2 Products

Product Documents for Histone Deacetylase 2/HDAC2 Antibody (OTI7E10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Histone Deacetylase 2/HDAC2 Antibody (OTI7E10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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