Histone Deacetylase 2/HDAC2 Antibody (S06-4E4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14893

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S06-4E4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone Deacetylase 2/HDAC2 Antibodies »

Product Summary for Histone Deacetylase 2/HDAC2 Antibody (S06-4E4)

Immunogen

Recombinant protein of human Histone Deacetylase 2/HDAC2 (Uniprot#: Q92769)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone Deacetylase 2/HDAC2 Antibody (S06-4E4)

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (S06-4E4) [NBP3-14893]

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (S06-4E4) [NBP3-14893]

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (S06-4E4) [NBP3-14893] - Western blot detection of Histone Deacetylase 2/HDAC2 in K562, C6, 3T3 cell lysates using Histone Deacetylase 2/HDAC2 Rabbit mAb (1:1000 diluted). Predicted band size: 55kDa. Observed band size: 60kDa.
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (S06-4E4) [NBP3-14893]

Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (S06-4E4) [NBP3-14893]

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (S06-4E4) [NBP3-14893] - Immunofluorescence of Histone Deacetylase 2/HDAC2 (green) in Hela cells using Histone Deacetylase 2/HDAC2 Rabbit mAb at dilution 1/200, and DAPI (blue)
Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (S06-4E4) [NBP3-14893]

Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (S06-4E4) [NBP3-14893]

Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (S06-4E4) [NBP3-14893] - Immunohistochemistry of Histone Deacetylase 2/HDAC2 in paraffin-embedded Human colon cancer tissue using Histone Deacetylase 2/HDAC2 Rabbit mAb at dilution 1/1

Applications for Histone Deacetylase 2/HDAC2 Antibody (S06-4E4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/200

Immunohistochemistry

1/500

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 2/HDAC2

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

Long Name

Histone Deacetylase 2

Alternate Names

HDAC2, YAF1

Gene Symbol

HDAC2

Additional Histone Deacetylase 2/HDAC2 Products

Product Documents for Histone Deacetylase 2/HDAC2 Antibody (S06-4E4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone Deacetylase 2/HDAC2 Antibody (S06-4E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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