Histone H1 Antibody (1415-1 (21NC85))
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65220
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1415-1 (21NC85)
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The exact immunogen is proprietary, but is composed of lysed nuclei of myeloid leukemia biopsy cells.
Specificity
This antibody recognises an antigen found in the nucleus. Antibody also preferentially reacts with histone H1/ DNA nucleosome as shown by ELISA and western blotting. In WB using non-reduced samples it recognises primarily a 30-32kDa histone H1 band, but other bands are present as well. This antibody stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Intense diffuse staining is obtained in interphase cells. The epitope has not been mapped.
Marker
Nuclear Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Histone H1 Antibody (1415-1 (21NC85))
![Immunohistochemistry: Histone H1 Antibody (21NC85) [NB100-65220]](https://resources.bio-techne.com/images/products/Histone-H1-Antibody-21NC85-Immunohistochemistry-NB100-65220-img0002.jpg "Immunohistochemistry: Histone H1 Antibody (21NC85) [NB100-65220]")
Immunohistochemistry: Histone H1 Antibody (21NC85) [NB100-65220]
Immunohistochemistry: Histone H1 Antibody (21NC85) [NB100-65220] - FFPE pig heart stained with NB100-65220 at 1:500.Applications for Histone H1 Antibody (1415-1 (21NC85))
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:20-1:50
Immunohistochemistry-Paraffin
1:500
Western Blot
1:100-1:2000
Application Notes
Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, ELISA and Western Blot
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NB100-65220 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.1% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H1
Alternate Names
H1 histone family, member 1, H1.1, H1A, H1F1HIST1, histone 1, H1a, histone cluster 1, H1a, histone H1.1, MGC126642, MGC138345
Gene Symbol
H1-1
Additional Histone H1 Products
Product Documents for Histone H1 Antibody (1415-1 (21NC85))
Product Specific Notices for Histone H1 Antibody (1415-1 (21NC85))
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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