Histone H1.3 Antibody (SR2420)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27720

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-27720
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2420

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H1.3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human Histone H1.3 (Uniprot #: P16402)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H1.3 Antibody (SR2420)

Immunocytochemistry/ Immunofluorescence: Histone H1.3 Antibody (SR2420) [NBP3-27720] -

Immunocytochemistry/ Immunofluorescence: Histone H1.3 Antibody (SR2420) [NBP3-27720] - Immunocytochemistry analysis of Histone H1.3 (green) in Hela using NBP3-27720, and DAPI(blue)

Immunohistochemistry-Paraffin: Histone H1.3 Antibody (SR2420) [NBP3-27720] -

Immunohistochemistry-Paraffin: Histone H1.3 Antibody (SR2420) [NBP3-27720] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western Blot: Histone H1.3 Antibody (SR2420) [NBP3-27720] -

Western Blot: Histone H1.3 Antibody (SR2420) [NBP3-27720] - Western blot analysis of Histone H1.3 in C6, 3T3, Hela lysates

Applications for Histone H1.3 Antibody (SR2420)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H1.3

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The histone H1.3 protein is encoded by the HIST1H1D gene and is 221 amino acids long at 22 kDA. H1 histones are critical for the condensation of nucleosome chains into higher order structures. HIST1H1D participates in PKA signaling, cell cycle start of DNA replication in early S phase, apoptosis, and histone modification and is known to interact with genes LMNA, EP300, SUMO1, NOP56 and YWHAQ. Researchers have investigated its role in treacher collins syndrome, meningioma, typhoid fever, and crohn's disease.

Alternate Names

H1 histone family, member 3, H1.3, H1F3H1D, histone 1, H1d, histone cluster 1, H1d, histone H1.3, Histone H1c, MGC138176

Gene Symbol

H1-3

Additional Histone H1.3 Products

Product Documents for Histone H1.3 Antibody (SR2420)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H1.3 Antibody (SR2420)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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