Histone H1.4 [p Thr17] Antibody (3E1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26420

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26420-100ul
NBP3-26420-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3E1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H1.4 Antibodies »

Product Specifications

Immunogen

Synthesized peptide [UniProt P10412]

Modification

p Thr 17

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H1.4 [p Thr17] Antibody (3E1)

Histone H1.4 [p Thr17] Antibody (3E1)

Immunohistochemistry: Histone H1.4 [p Thr17] Antibody (3E1) [NBP3-26420] -

Immunohistochemistry: Histone H1.4 [p Thr17] Antibody (3E1) [NBP3-26420] - Image of Histone H1.4 [p Thr17] Antibody (3E1) diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Histone H1.4 [p Thr17] Antibody (3E1)

Immunohistochemistry: Histone H1.4 [p Thr17] Antibody (3E1) [NBP3-26420] -

Immunohistochemistry: Histone H1.4 [p Thr17] Antibody (3E1) [NBP3-26420] - Image of Histone H1.4 [p Thr17] Antibody (3E1) diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Histone H1.4 [p Thr17] Antibody (3E1)

Immunocytochemistry/Immunofluorescence: Histone H1.4 [p Thr17] Antibody (3E1) [NBP3-26420] -

Immunocytochemistry/Immunofluorescence: Histone H1.4 [p Thr17] Antibody (3E1) [NBP3-26420] - Staining of MCF-7 cells with Histone H1.4 [p Thr17] Antibody (3E1) at 1:56, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for Histone H1.4 [p Thr17] Antibody (3E1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:30-1:200

Immunohistochemistry

1:50-1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Histone H1.4

The HIST1H1E gene encodes a 219 amino acid long, 21 kDA Histone H1.4 that functions by binding to linker DNA between nucleosome creating the macromolecular structure chromatin fiber. H1 histones as especially critical in condensation of nucleosome chains and the monitoring of individual gene transcription. HIST1H1E participates in PKA signaling, cell cycle start of DNA replication in early S phase, apoptosis, and histone modification. It interacts with genes HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4L. HIST1H1E has been researched regarding its role in progressive supranuclear palsy, pancreatitis, hepatitis, and x inactivation.

Alternate Names

dJ221C16.5, H1 histone family, member 4, H1.4, H1F4H1E, histone 1, H1e, histone cluster 1, H1e, histone H1.4, Histone H1b, MGC116819

Gene Symbol

H1-4

Additional Histone H1.4 Products

Product Documents for Histone H1.4 [p Thr17] Antibody (3E1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H1.4 [p Thr17] Antibody (3E1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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