Histone H1.4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-55150

Novus Biologicals, part of Bio-Techne
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NBP1-55150
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Histone H1.4 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to HIST1H1E(histone cluster 1, H1e) Antibody(against the N terminal of HIST1H1E (NP_005312). Peptide sequence MSETAPAAPAAPAPAEKTPVKKKARKSAGAAKRKASGPPVSELITKAVAA. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Histone H1.4 Antibody

Western Blot: Histone H1.4 Antibody [NBP1-55150]

Western Blot: Histone H1.4 Antibody [NBP1-55150]

Western Blot: Histone H1.4 Antibody [NBP1-55150] - ACHN cell lysate, concentration 0.2-1 ug/ml.

Applications for Histone H1.4 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H1.4

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Alternate Names

dJ221C16.5, H1 histone family, member 4, H1.4, H1F4H1E, histone 1, H1e, histone cluster 1, H1e, histone H1.4, Histone H1b, MGC116819

Entrez Gene IDs

3008 (Human)

Gene Symbol

H1-4

UniProt

P10412 (Human)

Additional Histone H1.4 Products

Product Documents for Histone H1.4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H1.4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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